The three typical aspartic proteinase genes ofArabidopsis thalianaare differentially expressed
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چکیده
منابع مشابه
Identification of Novel Placentally Expressed Aspartic Proteinase in Humans
This study presents pioneering data concerning the human pregnancy-associated glycoprotein-Like family, identified in the genome, of the term placental transcriptome and proteome. RNA-seq allowed the identification of 1364 bp hPAG-L/pep cDNA with at least 56.5% homology with other aspartic proteinases (APs). In silico analyses revealed 388 amino acids (aa) of full-length hPAG-L polypeptide prec...
متن کاملFinding Significantly Differentially Expressed Genes
Scientists are interested in how gene expression levels may vary under different treatments. They want to know which genes are expressed differently under treatment conditions and control conditions. Gene expression microarray data are modeled to find significant changes in gene expression levels between a control and treatment tissue co-hybridized on one slide. The model used is the Bayesian h...
متن کاملHunting for differentially expressed genes.
Differentially expressed genes are usually identified by comparing steady-state mRNA concentrations. Several methods have been used for this purpose, including differential hybridization, cDNA subtraction, differential display and, more recently, DNA chips. Subtractive hybridization has significantly improved after the polymerase chain reaction was incorporated into the original method and many...
متن کاملThe diversity and evolutionary relationships of the pregnancy-associated glycoproteins, an aspartic proteinase subfamily consisting of many trophoblast-expressed genes.
The pregnancy-associated glycoproteins (PAGs) are structurally related to the pepsins, thought to be restricted to the hooved (ungulate) mammals and characterized by being expressed specifically in the outer epithelial cell layer (chorion/trophectoderm) of the placenta. At least some PAGs are catalytically inactive as proteinases, although each appears to possess a cleft capable of binding pept...
متن کاملEffect of N-linked glycosylation on the aspartic proteinase porcine pepsin expressed from Pichia pastoris.
A study was undertaken to examine the effects of N-linked glycosylation on the structure-function of porcine pepsin. The N-linked motif was incorporated into four sites (two on the N-terminal domain and two on the C-terminal domain), and the recombinant protein expressed using Pichia pastoris. All four N-linked recombinants exhibited similar secondary and tertiary structure to nonglycosylated p...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 2002
ISSN: 0014-2956
DOI: 10.1046/j.1432-1033.2002.03168.x